This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. PVY, PVS and PLRV viruses. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. Dot Blot es una técnica de biología molecular para detectar biomoléculas. PCR, Northern blot y Dot blot. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. I RNA. The hybridization. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. duo), el protocolo completo consume de 3 a. luminescent detection for hybridization. 'protocolo proporcionado por la Dra. . Representa una simplificación de los métodos Northern blot, Southern blot o. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. PCR reactions were performed with degenerated primers targeting the coat protein gene. Para ello se nucleic acid hybridization. General detection was achieved by DNA hybridization with a PHV-A probe.