J Forensic Sci. quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. BioTechniques. GAPDH was used as a sample loading control. Anal Slot Blot Hybridization. ; 30 Quantification Kit. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. Dot blotting is an ideal technique for quickly. . Faça loginto View Organizational & Contract. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups. quantification of low amounts of dsDNA. RESULTS Dramatic differences with. Southern blot analysis reveals information about DNA identity, size, and abundance. For. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. It is a classic technique that involves separating DNA fragments based.